• To determine the time required

  2018-10-22

  

  To determine the time required for accurate viability assessment, approximately 1000 A. caninum eggs were transferred into 1.5mL centrifuge tubes, suspended in 1mL Phosphate Buffer Saline (PBS), and heat-inactivated on a heating block for 30min at 80°C. Tubes were centrifuged at 800× for 3min, and t

• In this laboratory based study fewer C parvum oocysts parall

  2018-10-22

  

  In this laboratory-based study, 60% fewer C. parvum oocysts paralleled by 80.3% lower turbidity was obtained following 90 min of sedimentation in wastewater treated with 4 mL L of 5% MO seed extract compared with untreated wastewater. This documented effect of increased sedimentation of oocysts in w

• PD98059 Damascenone Fig and ionone Fig

  2018-10-22

  

  β-Damascenone (Fig. 3) and β-ionone (Fig. 4a) are two representative aromas formed from carotenoid degradation. β-damascenone has an apple-like flavor and has an extremely low threshold in water (0.002ppb). It was first identified in Bulgarian rose oil in 1970 [9] and is an essential odor in black t

• ddPCR is claimed to possess advantages

  2018-10-22

  

  ddPCR is claimed to possess advantages over qPCR, including absolute quantification, unparalleled precision, increased signal-to-noise ratio, removal of PCR bias, reduced consumable costs, lower equipment costs and superior partitioning. However, ddPCR does have a limitation in quantifying high numb

• The use of MaSCs from GFP mice

  2018-10-22

  

  The use of MaSCs from GFP mice for in vivo transplant into wild type recipients enabled us to examine the purity of FACS-gated basal and luminal cell populations from the regenerated GFP glands. All epithelial order buprenorphine hydrochloride (GFP positive) were converged in the CD24+ population,

• br Materials and methods br Acknowledgments br

  2018-10-22

  

  Materials and methods Acknowledgments Introduction Human bone marrow stromal cells (HBMSCs) have therapeutic potential in cell transplantation and tissue engineering. In addition to their anti-inflammatory and immunomodulatory characteristics (Lee et al., 2009a; Casiraghi et al., 2010), the

• Thus with these data we assert that

  2018-10-22

  

  Thus, with these data we assert that small replicating cells do not “become” large replicating cells upon culture expansion of BMSCs. Nor are those large cells “old”, in the sense that these cells are from earlier generations that gradually increase in cell size with time in vitro. Rather, large cel

• Fibroblasts can be directly reprogrammed into neurons Vierbu

  2018-10-22

  

  Fibroblasts can be directly reprogrammed into neurons (Vierbuchen et al., 2010), cardiomyocytes (Ieda et al., 2010), and NSCs (Han et al., 2012) using tissue-specific combinations of transcription factors. However, the pluripotent reprogramming cocktail of Oct4, Sox2, Klf4, and Myc can also convert

• The production of new hair cells from

  2018-10-22

  

  The production of new hair NVP-TNKS656 from supporting cells could be increased by inhibition of Notch signaling in the damaged cochlea. We found, by the use of lineage tracing and confocal microscopy in the newborn cochlea after damage and Notch inhibition, that the capacity for supporting cell tr

• We observed a lower percentage of GFP

  2018-10-22

  

  We observed a lower percentage of GFP+ JWH 073 under standard differentiation conditions compared to the J1 Lhx6::GFP line (Maroof et al., 2010). We repeated the differentiations with another Lhx6::GFP+ Ainv15-derived clone (Ainv15.16) and found similarly low numbers of GFP+ cells at DD12 in the ab

• Our data in human PSC differ from Jang H

  2018-10-22

  

  Our data in human PSC differ from Jang H. et al.\'s results in mouse PSC (Jang et al., 2012). Indeed, in this particular publication, the authors showed that a high level of O-GlcNAcylation maintains mESC pluripotency state and inhibits differentiation for all germ layers. Our results suggest a more

• Potential candidates contributing to the fate determination

  2018-10-22

  

  Potential candidates contributing to the fate determination in the injured spinal cord include bone morphogenetic proteins (BMPs) — in particular BMP2 and BMP4, which strongly promote astroglial differentiation at the expense of oligodendroglial and neuronal differentiation (Bonaguidi et al., 2005;

• KRAB associated protein KAP also known as TRIM and TIF

  2018-10-22

  

  KRAB-associated protein 1 (KAP1, also known as TRIM28 and TIF1β) is a novel Oct3/4-dependent transcription factor that regulates pluripotency (Seki et al., 2010). KAP1 was initially identified as an interaction partner of KRAB-domain containing zinc finger transcription factors (Friedman et al., 199

• The identification of progenitor populations in

  2018-10-20

  

  The identification of progenitor populations in the adult heart adds increased complexity to heart development, and raises many interesting questions – for example, are fetal or adult CPCs remnants of earlier embryonic progenitors, have they migrated in from other sources, or have they arisen de nov

• The karyotype of the BIOT LOAD iPSC

  2018-10-20

  

  The karyotype of the BIOT-4828-LOAD iPSC line was determined with Giemsa-banding, proving normal diploid 46, XX karyotype, without any detectable abnormalities (Fig. 1B). Expression of pluripotency markers was examined by immunocytochemistry staining, using order UNC2025 against human OCT3/4, E-CA

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