The pluripotency of MPSII line was confirmed by alkaline

2018-10-20

The pluripotency of MPSII-4.1 line was confirmed by alkaline phosphatase staining (ALP) and by immunocytochemistry staining (ICC) for endogenous NANOG and -CADHERIN (B) after silencing of the exogenous hOKSM construct. The spontaneous differentiation potential of the iPSC line towards the three ge

In the present study we investigated

2018-10-20

In the present study, we investigated the function of FOXO3 in adult SCs using an inducible genetic system to ablate the Foxo3 gene specifically in SCs. We found that FOXO3 is required for the self-renewal of SCs during muscle regeneration. FOXO3-deficient SCs proliferate more rapidly than control S

Another major limitation of the adult

2018-10-20

Another major limitation of the adult cardiac stem cell field is the inability to get full differentiation from putative stem/progenitor histamine inhibitors in vitro or in vivo. In fact, although expression of cardiac genes and proteins is demonstrated frequently, the levels, relative to authentic

br Results br Discussion We highlight the generation of

2018-10-20

Results Discussion We highlight the generation of human functional haploid spermatids from human SSCs of cryptorchid patients. Cryptorchidism is the most common etiologic factor for azoospermia in adults. Among men with untreated bilateral cryptorchidism, 89% eventually develop azoospermia (Ch

Since the discovery of these cells Ingram

2018-10-20

Since the discovery of these MLN9708 manufacturer (Ingram et al., 2004), secondary colonies have been generated in several studies to assess the immaturity of ECFCs from blood or vascular wall and hierarchize them (Alphonse et al., 2015; Ingram et al., 2005; Patel et al., 2016). The aim of this wor

Alternative approaches to LV are non viral vectors

2018-10-20

Alternative approaches to LV are non-viral vectors such as RNA or DNA, which are considered safer due to their integration-free nature (Hardee et al., 2017). However, manufacturing RNA is expensive and difficult because RNA has a propensity to degrade (Yin et al., 2014). On the other hand, DNA plasm

Expression profiles from tumor tissue and derived cell lines

2018-10-20

Expression profiles from tumor tissue and derived cell lines are extremely different, thus the current transcriptomic signature for GBM subtyping may not apply in vitro. Using our precursor state signature, we were able to identify an association with the proneural and mesenchymal subtypes. Interest

br Results br Discussion Our analysis of satellite

2018-10-20

Results Discussion Our analysis of satellite cell frequency in muscles of the head, trunk, and limbs suggests considerable homogeneity among different healthy human muscle types. However, when considering muscle volume, higher satellite cell content of the temporalis may relate to physiologica

In addition to studying drug effects on AP and calcium

2018-10-20

In addition to studying drug effects on AP and calcium handling, we also showed the potential of using genetic reporters for studying inherited arrhythmogenic disorders. To this end, we utilized a previously established LQTS2 patient-specific line (Itzhaki et al., 2011a) and also generated a new hiP

Because freshly isolated hepatocytes rapidly lose their func

2018-10-20

Because freshly isolated hepatocytes rapidly lose their functions, it is a major challenge to generate fully functional hepatocytes from pluripotent stem cells. While hepatocytes derived from CPM+ LPCs expressed high levels of metabolic activity, the levels of some proteins are not as high as primar

However PSCs have major safety concerns such as teratoma

2018-10-20

However, PSCs have major safety concerns, such as teratoma formation due to possible contamination of the residual undifferentiated PSCs (Lee et al., 2013a). Teratoma is a unique characteristic of PSCs owing to their pluripotency and ability to undergo unlimited proliferation (Gruen and Grabel, 200

The role of Wnt catenin signaling in reprogramming

2018-10-20

The role of Wnt/β-catenin signaling in reprogramming has also been investigated. Exogenously introduced WNT3A enhances fibroblast reprogramming in the absence of c-Myc (Marson et al., 2008). Knockdown or knockout of T cell factors or treatments with several drugs that control the Wnt pathway can cha

br Results br Discussion The

2018-10-20

Results Discussion The range of cell types in Haemopedia makes identification of lineage-specific genes more rigorous. The genes that we have identified as lineage signatures are highly specific to each lineage and are lineage specific in other datasets (Figure S5). This provides a powerful re

Given that the ECM becomes more

2018-10-20

Given that the ECM becomes more complex as ECs differentiate, it is of particular interest to understand the outside-in signaling that might modulate their behavior. Similar to other cell types, ECs interact with their environment in large part via the integrin family of adhesion receptors that cons

Hello world!

2018-07-29