• KRAB associated protein KAP also known as TRIM and TIF

  2018-10-22

  

  KRAB-associated protein 1 (KAP1, also known as TRIM28 and TIF1β) is a novel Oct3/4-dependent transcription factor that regulates pluripotency (Seki et al., 2010). KAP1 was initially identified as an interaction partner of KRAB-domain containing zinc finger transcription factors (Friedman et al., 199

• The identification of progenitor populations in

  2018-10-20

  

  The identification of progenitor populations in the adult heart adds increased complexity to heart development, and raises many interesting questions – for example, are fetal or adult CPCs remnants of earlier embryonic progenitors, have they migrated in from other sources, or have they arisen de nov

• The karyotype of the BIOT LOAD iPSC

  2018-10-20

  

  The karyotype of the BIOT-4828-LOAD iPSC line was determined with Giemsa-banding, proving normal diploid 46, XX karyotype, without any detectable abnormalities (Fig. 1B). Expression of pluripotency markers was examined by immunocytochemistry staining, using order UNC2025 against human OCT3/4, E-CA

• The pluripotency of MPSII line was confirmed by alkaline

  2018-10-20

  

  The pluripotency of MPSII-4.1 line was confirmed by alkaline phosphatase staining (ALP) and by immunocytochemistry staining (ICC) for endogenous NANOG and -CADHERIN (B) after silencing of the exogenous hOKSM construct. The spontaneous differentiation potential of the iPSC line towards the three ge

• In the present study we investigated

  2018-10-20

  

  In the present study, we investigated the function of FOXO3 in adult SCs using an inducible genetic system to ablate the Foxo3 gene specifically in SCs. We found that FOXO3 is required for the self-renewal of SCs during muscle regeneration. FOXO3-deficient SCs proliferate more rapidly than control S

• Another major limitation of the adult

  2018-10-20

  

  Another major limitation of the adult cardiac stem cell field is the inability to get full differentiation from putative stem/progenitor histamine inhibitors in vitro or in vivo. In fact, although expression of cardiac genes and proteins is demonstrated frequently, the levels, relative to authentic

• br Results br Discussion We highlight the generation of

  2018-10-20

  

  Results Discussion We highlight the generation of human functional haploid spermatids from human SSCs of cryptorchid patients. Cryptorchidism is the most common etiologic factor for azoospermia in adults. Among men with untreated bilateral cryptorchidism, 89% eventually develop azoospermia (Ch

• Since the discovery of these cells Ingram

  2018-10-20

  

  Since the discovery of these MLN9708 manufacturer (Ingram et al., 2004), secondary colonies have been generated in several studies to assess the immaturity of ECFCs from blood or vascular wall and hierarchize them (Alphonse et al., 2015; Ingram et al., 2005; Patel et al., 2016). The aim of this wor

• Alternative approaches to LV are non viral vectors

  2018-10-20

  

  Alternative approaches to LV are non-viral vectors such as RNA or DNA, which are considered safer due to their integration-free nature (Hardee et al., 2017). However, manufacturing RNA is expensive and difficult because RNA has a propensity to degrade (Yin et al., 2014). On the other hand, DNA plasm

• Expression profiles from tumor tissue and derived cell lines

  2018-10-20

  

  Expression profiles from tumor tissue and derived cell lines are extremely different, thus the current transcriptomic signature for GBM subtyping may not apply in vitro. Using our precursor state signature, we were able to identify an association with the proneural and mesenchymal subtypes. Interest

• br Results br Discussion Our analysis of satellite

  2018-10-20

  

  Results Discussion Our analysis of satellite cell frequency in muscles of the head, trunk, and limbs suggests considerable homogeneity among different healthy human muscle types. However, when considering muscle volume, higher satellite cell content of the temporalis may relate to physiologica

• In addition to studying drug effects on AP and calcium

  2018-10-20

  

  In addition to studying drug effects on AP and calcium handling, we also showed the potential of using genetic reporters for studying inherited arrhythmogenic disorders. To this end, we utilized a previously established LQTS2 patient-specific line (Itzhaki et al., 2011a) and also generated a new hiP

• Because freshly isolated hepatocytes rapidly lose their func

  2018-10-20

  

  Because freshly isolated hepatocytes rapidly lose their functions, it is a major challenge to generate fully functional hepatocytes from pluripotent stem cells. While hepatocytes derived from CPM+ LPCs expressed high levels of metabolic activity, the levels of some proteins are not as high as primar

• However PSCs have major safety concerns such as teratoma

  2018-10-20

  

  However, PSCs have major safety concerns, such as teratoma formation due to possible contamination of the residual undifferentiated PSCs (Lee et al., 2013a). Teratoma is a unique characteristic of PSCs owing to their pluripotency and ability to undergo unlimited proliferation (Gruen and Grabel, 200

• The role of Wnt catenin signaling in reprogramming

  2018-10-20

  

  The role of Wnt/β-catenin signaling in reprogramming has also been investigated. Exogenously introduced WNT3A enhances fibroblast reprogramming in the absence of c-Myc (Marson et al., 2008). Knockdown or knockout of T cell factors or treatments with several drugs that control the Wnt pathway can cha

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